In order to evaluating the effect of flooding on growth and biological nitrogen fixation (BNF) in SUBTERRANEAN clover, an experiment was carried out using a randomized complete blocks with 4 replications and 2 treatments. Treatments were normal conditions of Gorgan and artificial flooding condition. Dactylis gelomerata was used as a reference plant to estimate BNF in SUBTERRANEAN clover. Result showed that flooding conditions reduced dry matter production, BNF, nodules fresh weight and leaf area by 14.6, 31.7, 28.7 and 14.4% in comparison to control. But, nitrogen yield was not significantly affected by flooding.